Chromatography Reagents & Solvents

1,2-Dimethoxyethane

HPLC Grade Solvents

1,2-Dimethoxyethane for HPLC, 99.9%, inhibitor-free 

 

grade  

for HPLC

vapor density  

3.1 (20 °C, vs air)

vapor pressure  

48 mmHg ( 20 °C)

InChI Key  

XTHFKEDIFFGKHM-UHFFFAOYSA-N

assay  

99.9%

autoignition temp.  

396 °F

purified by  

glass distillation

 

1-Chlorobutane

HPLC Grade Solvents

1-Chlorobutane for HPLC, ≥99.8% 

 

grade  

for HPLC

vapor density  

3.2 (vs air)

vapor pressure  

80.1 mmHg ( 78.4 °C)

InChI Key  

VFWCMGCRMGJXDK-UHFFFAOYSA-N

assay  

≥99.8%

autoignition temp.  

860 °F

expl. lim.  

10.1 %

DescriptionPackaging

1, 2, 4×4 L in glass bottle

Application

1-Chlorobutane may be used in the synthesis of ionic liquids, 1-butyl-3-methylimidazolium hydrogen sulfate ([Bmim]+[HSO4]-) and 1-butyl-3-methylimidazolium dihydrogen phosphate ([Bmim]+[H2PO4]-).

General description

1-Chlorobutane is an alkyl halide. It undergoes reaction with nanocrystalline MgO at 200-350°C to afford butane isomers and MgCl2, via dehydrochlorination.

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1-Methyl-2-Pyrrolidinone

Solvents for GC applications1-Methyl-2-pyrrolidinone for metal speciation analysis, ≥99.0% (GC) 

 

vapor density  

3.4 (vs air)

vapor pressure  

0.29 mmHg ( 20 °C)

 

0.99 mmHg ( 40 °C)

InChI Key  

SECXISVLQFMRJM-UHFFFAOYSA-N

assay  

≥99.0% (GC)

autoignition temp.  

518 °F

quality  

for metal speciation analysis

DescriptionApplication

NMP was used:
• as an aqueous physical solvent to measure the molar heat capacities
• in the synthesis of Nafion/SiO2 nanocomposite membranes.
• in the synthesis of N-Butyl-N,N-dimethyl chitosan iodide (QCh).
• as an inducer to demonstrate phenotypic resistance to Chloramphenicol and ampicillin in Escherichia coli K-12 starins.
• used as polar solvent inducer for erythroleukemic cell differentiation.
• as a blocking reagent in microarray manufacturing.

General description

NMP, effective solvent for organic and inorganic compounds. Tertiary amides in H-bond basicity are decreased by bulky substituents on carbon than bulky substituents on nitrogen. It is also an effective solvent for the decarbonylation of aldehydes. It exhibits as reagent and amidines formed from NMP exist as amidine-enediamine tautomers. As cosolvent it has ability to enhance the reactivity of other reagents.

 

 

1-Propanol

HPLC Grade Solvents

grade  

for HPLC

vapor density  

2.1 (vs air)

vapor pressure  

10 mmHg ( 147 °C)

 

14.9 mmHg ( 20 °C)

InChI Key  

BDERNNFJNOPAEC-UHFFFAOYSA-N

assay  

≥99.9%

autoignition temp.  

700 °F

DescriptionPackaging

1, 6×1, 2 L in glass bottle

100 mL in glass bottle

4 L in PVC-coated bottle

Application

1-Propanol may be employed as a solvent for the synthesis of mesoporous alumina.

General description

1-Propanol is a linear alcohol. It exhibits Debye-type relaxation process which ressembles the α-relaxation and Johari-Goldstein type β-relaxation features of the supercooled liquids (nonhydrogen-bonding type).It has been tested as a substitute of the fuel for various fuel cells. Its biosynthesis from glucose using Escherichia coli strain has been proposed.

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2,2,4-Trimethylpentane

HPLC Grade Solvents

grade  

for HPLC

vapor density  

3.9 (vs air)

vapor pressure  

41 mmHg ( 21 °C)

InChI Key  

NHTMVDHEPJAVLT-UHFFFAOYSA-N

assay  

≥99%

autoignition temp.  

745 °F

expl. lim.  

6 %

DescriptionPackaging

1, 6×1, 2, 4×2, 4×4 L in glass bottle

100 mL in glass bottle

Application

2,2,4-Trimethylpentane (TMP, Isooctane) has been used for the isolation of mercury species during the HPLC cold vapor atomic fluorescence spectrometric (HPLC-CVAFS) quantification of MeHg in various biological samples.It may be used to evaluate various physical properties (densities, viscosities, refractive indexes and surface tensions) of the binary mixtures of TMP and cyclohexane.

General description

2,2,4-Trimethylpentane (TMP, Isooctane) is a saturated, branched hydrocarbon.It has been reported to be nephrotoxic to male rats. Density and viscosity of TMP at various temperatures (198K - 348K) and pressures (100MPa) has been evaluated. It has been indentified as nephrotoxicity inducing component in unleaded gasoline.

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2-Propanol

Solvents for GC applications

2-Propanol for gas chromatography ECD and FID SupraSolv®. CAS No. 67-63-0, EC Number 200-661-7., for gas chromatography ECD and FID SupraSolv® 

packaging  

1 l in Glass bottle (1009981000)

 

2.5 l in Glass bottle (1009982500)

bp  

82.4 °C (1013 hPa)

expl. lim.    

2 - 13.4 %(V)

vapor pressure  

43 hPa (20 °C)

density  

0.786 g/cm3 (20 °C)

flash point  

12 °C

DescriptionOther Notes

SupraSolv® ECD and FID is specially developed for gas chromatography in combination with ECD (Electron Capture Detetor) and FID (Flame Ionization detector). SupraSolv® solvents offer the largest specified retention time range, a clear baseline and a minimal signal-to-noise ratio leading to reliable and reproducible analysis results.

 

Acetic Acid 100%

HPLC Buffers and Reagents 

Acetic acid 100% for HPLC LiChropur®

 

packaging  

100 ml in Glass bottle (5438080100)

bp  

116 - 118 °C (1013 hPa)

expl. lim.    

4 - 19.9 %(V)

vapor pressure  

15.4 hPa (20 °C)

solubility  

602.9 g/l

density  

1.05 g/cm3 (20 °C)

flash point  

39 °C

Acetone

Solvents for GC applications

Acetone for gas chromatography ECD and FID SupraSolv®. Acetone chemical formula CH₃COCH₃, molar mass 58.08 g/mol and CAS No. 67-64-1., for gas chromatography ECD and FID SupraSolv® 

 

packaging  

1 l in Glass bottle (1000121000)

 

2.5 l in Glass bottle (1000122500)

 

30 l in Barrel stainl. st. (1000129030)

 

4 l in Glass bottle (1000124000)

bp  

56.2 °C (1013 hPa)

expl. lim.    

2.6 - 12.8 %(V)

vapor pressure  

233 hPa (20 °C)

Acetonitrile

 Solvents for GC applications

 

grade  

for mass spectrometry

vapor density  

1.41 (vs air)

vapor pressure  

72.8 mmHg ( 20 °C)

InChI Key  

WEVYAHXRMPXWCK-UHFFFAOYSA-N

autoignition temp.  

973 °F

expl. lim.  

16 %

refractive index  

n20/D 1.344(lit.)

Description

Packaging

1 L in glass bottle

100, 250 mL in glass bottle

Acetonitrile ( HPLC-GC)

HPLC Grade Solvents

 

Acetonitrile for HPLC-GC, ≥99.8% (GC) 

 

 

grade  

for HPLC-GC

vapor density  

1.41 (vs air)

vapor pressure  

72.8 mmHg ( 20 °C)

InChI Key  

WEVYAHXRMPXWCK-UHFFFAOYSA-N

assay  

≥99.8% (GC)

autoignition temp.  

973 °F

expl. lim.  

16 %

Ammonia Solution 25%

HPLC Buffers and Reagents 

 

Ammonia solution 25%

for HPLC LiChropur® 

 

packaging  

100 ml in Glass bottle (5438300100)

bp  

37.7 °C (1013 hPa)

expl. lim.    

15.4 - 33.6 %(V)

vapor pressure  

483 hPa (20 °C)

density  

0.903 g/cm3 (20 °C)

mp  

-57.5 °C

storage temp.  

Store at +2°C to +25°C.

Ammonium Acetate

HPLC Buffers and Reagents 

Ammonium acetate for HPLC LiChropur®

 

packaging  

100 g in Plastic bottle (5438340100)

solubility  

1480 g/l

density  

1.17 g/cm3 (20 °C)

mp  

114 °C

pH  

6.7 - 7.3 (50 g/l, H₂O, 25 °C)

bulk density  

410 kg/m3

shipped in  

ambient

 

Benzonitrile

HPLC Grade Solvents

 

 

Grade  

for HPLC

InChI Key  

JFDZBHWFFUWGJE-UHFFFAOYSA-N

assay  

99.9%

purified by  

glass distillation

impurities  

≤0.03% water

evapn. residue  

<0.0005%

refractive index  

n20/D 1.528(lit.)

Bispyrazolone

TLC Reagents 

 

Bispyrazolone for TLC derivatization, for the det.of cyanide, ≥98.0% 
grade  for TLC derivatization
InChI Key  FORCWSNQDMPPOC-UHFFFAOYSA-N
assay  ≥98.0% (TLC)
 ≥98.0%
quality  for the det.of cyanide
solubility  formic acid: 50 mg/mL, clear, yellow
Other Notes

Determination of ammonia; Assay of cyanogenic compounds

Application

Bispyrazolone was used in an experimental procedure, done in order to remove linamarin in starch from cassava roots, by using plant cell wall-degrading enzymes, xylanase and cellulase. It was also used for spectrophotometric determination and quantization of urea in urine samples.


Bromothymol Blue

TLC Reagents

 

description  

Bromothymol Blue reagent

InChI Key  

NUHCTOLBWMJMLX-UHFFFAOYSA-N

usage  

the detection of L/S ratio lipids

packaging  

pkg of 200 mL

mp  

200-202 °C(lit.)

Application

Bromothymol blue may be used to adjust pH between 7 and 8 of the sample during a gas chromatographic analysis to estimate urinary oxalate.

General description

Bromothymol blue is a pH indicator which is sparingly soluble in water, benzene but is soluble in ethanol. It is insoluble in petroleum ether. It has its major uses in Sol-gel matrix, fuel cells, optical sensors, combustion gas detection system. It is considered as a toxic reagent.

Chromatofocusing Reagents

pH range  

8.0 - 10.5

capacity  

0.35 mmol/pH·mL buffering capacity

storage temp.  

2-8°C

Other Notes

IEF carrier ampholytes

Biochem/physiol Actions

Ampholytes are used to generate a pH gradient under application of an electric field.

Legal Information

Pharmalyte is a registered trademark of GE Healthcare

Curcumin Solution

TLC Reagents

 ~0.1 % (w/v) (in ethanol with 2M HCl (99:1 v/v)), for TLC derivatization

grade  

for TLC derivatization

InChI Key  

VFLDPWHFBUODDF-FCXRPNKRSA-N

concentration  

~0.1 % (w/v) (in ethanol with 2M HCl (99:1 v/v))

refractive index  

n20/D 1.363

density  

0.794 g/mL at 20 °C

Application

Effective TLC derivatization reagent for the colorimetric detection of various boronic acids and their derivatives  


Cyclohexane - Ethyl Acetate

Solvents for GC applications

grade  

for pesticide residue analysis

assay  

49.5-50.5% (cyclohexane, GC)

 

49.5-50.5% (ethyl acetate, GC)

impurities  

≤0.01% water

 

≤5 ng/L lindane

evapn. residue  

≤0.001%

DescriptionApplication

Suitable for sample cleanup and/or extraction when performing pesticide residue analysis[1][2].

Legal Information

PESTANAL is a registered trademark of Sigma-Aldrich International GmbH 

Di-Sodium Hydrogen Phosphate Anhydrous

HPLC Buffers and Reagents 

packaging  

100 g in Plastic bottle (5438380100)

solubility  

77 g/l

mp  

250 °C

pH  

8.7 - 9.3 (10 g/l, H₂O, 20 °C)

bulk density  

880 kg/m3

shipped in  

ambient

storage temp.  

Store at +5°C to +30°C.

Ethyl Acetate

HPLC Grade Solvents

grade  

for HPLC

vapor density  

3 (20 °C, vs air)

vapor pressure  

73 mmHg ( 20 °C)

InChI Key  

XEKOWRVHYACXOJ-UHFFFAOYSA-N

assay  

≥99.8%

autoignition temp.  

801 °F

expl. lim.  

2.2-11.5 %, 38 °F

Formic Acid 98 - 100%

HPLC Buffers and Reagents



 Formic acid 98 - 100%

for HPLC LiChropur® 

 

packaging  

0.1 l in Glass bottle (5438040100)

bp  

101 °C (1013 hPa)

expl. lim.    

12 - 38 %(V)

vapor pressure  

42 hPa (20 °C)

density  

1.22 g/cm3 (20 °C)

flash point  

49.5 °C

mp  

4 °C

 

H2o

Solvents for GC applications 

Water solutioncontains 0.1 % (v/v) formic acid, for UHPLC, for mass spectrometry

 

grade  

for mass spectrometry

InChI Key  

XLYOFNOQVPJJNP-UHFFFAOYSA-N

contains  

0.1 % (v/v) formic acid

Heptane

HPLC Grade Solvents
grade  for HPLC
vapor density  3.5 (vs air)
vapor pressure  40 mmHg ( 20 °C)
 83 mmHg ( 37.7 °C)
InChI Key  IMNFDUFMRHMDMM-UHFFFAOYSA-N
assay  ≥99%
autoignition temp.  433 °F

Methanol (UHPLC)

Solvents for GC applications

Methanol UHPLC, for mass spectrometry 

 

grade  

UHPLC

 

for mass spectrometry

vapor density  

1.11 (vs air)

vapor pressure  

410 mmHg ( 50 °C)

 

97.68 mmHg ( 20 °C)

InChI Key  

OKKJLVBELUTLKV-UHFFFAOYSA-N

autoignition temp.  

725 °F


Ortho-Phosphoric Acid 85%

HPLC Buffers and Reagents 

ortho-Phosphoric acid 85%

for HPLC LiChropur® 

 

packaging  

100 ml in Plastic bottle (5438280100)

bp  

158 °C (1013 mbar)

vapor pressure  

2 hPa (20 °C)

density  

1.71 g/cm3 (20 °C)

mp  

21 °C

pH  

<0.5 (100 g/l, H₂O, 20 °C)

viscosity kinematic  

30.5 mm2/s (20 °C)

Potassium Bromide

TLC ReagentsPotassium bromide puriss. p.a., ACS reagent, ≥99.5% (AT) 

 Application

May be used for removal of peripheral membrane proteins.

Potassium bromide may be employed in the following studies:
• Spectrophotometric determination of aromatic amino compounds. 
• Preparation of AgBr nanoparticles (NPs).
• In combination with cerium(IV) ammonium nitrate (CAN), it has been employed for the bromination of alkenes.
• As an oxidant for the selective monobromination of various deactivated anilines.
• Preparation of sample pellets for IR spectroscopic investigations.

Other Notes

TLC detection, for the in situ production of bromine.

General description

Potassium bromide (KBr) is an alkaline-earth chloride. Density, viscosity and electrolytic conductivity of concentrated aqueous solutions of alkaline-earth chlorides [KBr, LaCl3, Na2SO4, NaBr, KNO3 and Cd(NO3)2] have been evaluated.

 

grade  ACS reagent
vapor pressure  <0.01 mmHg ( 20 °C)
 1 mmHg ( 795 °C)
InChI Key  IOLCXVTUBQKXJR-UHFFFAOYSA-M
grade  puriss. p.a.
assay  ≥99.5% (AT)
impurities  ≤0.001% total nitrogen (N)

 

Potassium Dihydrogen Phosphate Anhydrous

HPLC Buffers and Reagents

packaging  

100 g in Plastic bottle (5438410100)

solubility  

208 g/l

density  

2.33 g/cm3 (21.5 °C)

mp  

253 °C

pH  

4.2 - 4.6 (20 g/l, H₂O, 20 °C)

bulk density  

1200 kg/m3

shipped in  

ambient

 

Sodium Acetate Trihydrate

HPLC Buffers and Reagents 

Sodium acetate trihydrate for HPLC LiChropur® 

 

packaging  

100 g in Plastic bottle (5438330100)

solubility  

613 g/l

density  

1.42 g/cm3 (20 °C)

flash point  

>250 °C

autoignition temp.  

607 °C

mp  

58 °C

pH  

7.5 - 9.2 (50 g/l, H₂O, 20 °C)

 

 

Sodium Chloride

HPLC Buffers and Reagents 

Sodium chloride

for HPLC LiChropur® 

packaging  

100 g in Plastic bottle (5438320100)

bp  

1461 °C (1013 hPa)

vapor pressure  

1.3 hPa (865 °C)

solubility  

358 g/l

density  

2.17 g/cm3 (20 °C)

mp  

801 °C

pH  

4.5 - 7.0 (100 g/l, H₂O, 20 °C)

 

Sulfuric Acid 98%

HPLC Buffers and Reagents 

Sulfuric acid 98%

for HPLC LiChropur®

 

packaging  

100 ml in Glass bottle (5438270100)

bp  

335 °C (1013 hPa)

vapor pressure  

0.0001 hPa (20 °C)

density  

1.84 g/cm3 (20 °C)

mp  

-20 °C

pH  

0.3 (49 g/l, H₂O, 25 °C)

shipped in  

ambient

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